Figure 2

EBGP protected SOD1^G85R-induced neurotoxicity via the reduction of the aggregates of SOD1^G85R. (A) Representative fluorescent microscopy images of N2a cells expressing mCherry-SOD1^G85R incubated for 24 h with 20 ng/mL EBGP. The arrowheads indicate the intracellular SOD1 aggregates. (B) Quantified data of intracellular SOD1 aggregates are expressed as mean ± S.E.M from three independent experiments. In each experiment, at least 200 cells were counted. (C) Reduction of Triton-insoluble mutant SOD1^G85R by EBGP. After treatment of 20 ng/mL EBGP, N2a cells expressing SOD1^G85R were lysed with 1% TritonX-100. Triton-insoluble fraction was resuspended with 2% SDS and analyzed with immunoblotting. (D) The density of Triton-insoluble mutant SOD1^G85R is given as mean ± S.E.M from three independent experiment, based on the density of Triton-insoluble mutant SOD1^WT. (E) N2a cells expressing mCherry-SOD1^G85R were treated with 20 ng/mL EBGP. The cell viability was measured by MTT assay. ***p < 0.001. Scale bar: 50 µm.