Figure 3 | Scientific Reports

Figure 3

From: Lipopolysaccharides induced inflammatory responses and electrophysiological dysfunctions in human-induced pluripotent stem cell derived cardiomyocytes

Figure 3

LPS induced cell impairment. (A) ELISA analysis showing that 48 h-but not 6 h-treatment with LPS increased concentrations of human Troponin T (TNNT) in supernatants of LPS-treated hiPSC-CMs. p-values (test for linear trend): 6 h > 0.05, 48 h < 0.05. (B,C) FACS analysis for necrosis (7AAD positive) and apoptosis (Annexin-V positive) ratio of hiPS-CMs treated by LPS in different concentrations for 6 and 48 hours. 6 h-treatment showed a dose dependent increase of apoptotic cells ratio, but no influence on necrotic ratio (B). 48 h-treatment showed a dose dependent increase of necrotic cells ratio (C). The increase in apoptotic ratio was observed only when LPS concentration was raised to 100 µg/ml (p < 0.001 vs. 0 µg/ml). Test for linear trend: 6 h, necrosis, p > 0.05, apoptosis, p < 0.001; 48 h, necrosis, p = 0.003.

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