Table 2 Kinetic parameters for WT SCRII, SCRII-mtf and crosslinked SCRII towaids the asymmertric reduction of aryl ketones.
No | WT SCRII | SCRII-mtf | Crosslinked SCRII | ||||||
|---|---|---|---|---|---|---|---|---|---|
K m (mM) | k cat (s−1) | k cat/K m (s−1mM−1) | K m (mM) | k cat (s−1) | k cat/K m (s−1mM−1) | K m (mM) | k cat (s−1) | k cat/K m (s−1mM-1) | |
1a | 2.36 | 7.03 | 2.98 | 2.45 | 7.61 | 3.11 | 1.85 | 10.23 | 5.53 |
2a | 4.52 | 15.54 | 3.44 | 3.98 | 15.94 | 4.01 | 1.40 | 20.64 | 14.74 |
3a | 4.33 | 5.36 | 1.24 | 4.12 | 6.35 | 1.54 | 2.39 | 9.34 | 3.91 |
4a | 3.86 | 7.83 | 2.03 | 3.61 | 7.89 | 2.19 | 3.13 | 10.38 | 3.32 |
5a | 3.10 | 8.36 | 2.70 | 3.23 | 9.13 | 2.83 | 1.84 | 13.64 | 7.41 |
6a | — | — | — | — | — | — | — | — | — |
7a | 4.86 | 7.20 | 1.48 | 4.73 | 7.81 | 1.65 | 4.51 | 9.37 | 2.08 |
8a | 4.91 | 7.94 | 1.62 | 4.82 | 7.88 | 1.63 | 4.11 | 9.82 | 2.39 |
9a | 4.73 | 7.90 | 1.67 | 4.50 | 8.11 | 1.80 | 1.98 | 10.14 | 5.12 |
10a | 4.01 | 14.34 | 3.58 | 4.10 | 13.94 | 3.40 | 3.82 | 20.67 | 5.41 |
11a | 3.17 | 15.24 | 4.81 | 3.12 | 16.72 | 5.36 | 1.86 | 23.41 | 12.59 |
12a | 3.51 | 18.62 | 5.30 | 3.50 | 19.18 | 5.48 | 1.14 | 26.83 | 23.54 |
13a | — | — | — | — | — | — | — | — | — |
14a | 5.03 | 4.62 | 0.92 | 4.98 | 4.71 | 0.95 | 4.08 | 9.43 | 2.31 |
