Table 3 Specific activity and stereoselective efficiency of WT oxidoreductases and crosslinked oxidoreductases toward their favorite substrates.

From: Sortase A-mediated crosslinked short-chain dehydrogenases/reductases as novel biocatalysts with improved thermostability and catalytic efficiency

Enzymes

S

WT oxidoreductases

Crosslinked oxidoreductases

Activity a (μmol/(min·mg))

Cf b ee (%)

Yd b (%)

Activity a (μmol/(min·mg))

Cf b ee (%)

Ydb (%)

ADHR

10a

1.04

S(>99)

27

6.82

S(>99)

67

C1

13a

0.86

R(>99)

23

6.11

R(>99)

61

C2

13a

2.12

R(>99)

43

11.32

R(>99)

>99

CR2

1a

0.93

R(81)

25

5.86

R (83)

65

CR4

1a

5.81

R(>99)

79

36.81

R(>99)

>99

S1

11a

0.29

S(>99)

13

3.14

S(>99)

50

SCR1

2a

1.05

S(>99)

30

8.31

S(>99)

97

SCR3

2a

2.11

S(98)

46

14.20

S(99)

99

  1. aStandard assay conditions: 100 mM phosphate buffer (pH 7.0), 0.5 mM NADPH, 5 mM substrate, 1 mg/mL enzymes at 35 °C. The data was the representative of three independent experiments and standard errors were not more than 5%. bThe biotransformation was conducted with 5 g/L substrate and sufficient NADPH for 6 h.
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