Figure 6
From: How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways
YHC, GKW and GRA may all target on ERK-MAPK pathway. (A) Network of targets and pathways (including diseases) interact with YHC, GKW or GRA. Network pharmacology study identified 30 potential targets and 49 potential pathways for these compounds. The targets and pathways linked with GRA were mostly linked with GKW or YHC, which indicate tight interactions between YH and GC. Among the shared targets and pathways, MAP2K1 (equal to MEK1) and FGFR1 are the upstream factors of ERK-MAPK pathway (yellow nodes). This pathway mediates renal AQP 2 expression. Nodes of targets are blue, nodes of pathways are light green, edges linking molecules and targets are orange (YHC and GKW) or green (GRA), edges linking targets and pathways are blue. (B) Molecule docking images showing how YHC, GKW and GRA bind to the active domain of their targets, in each case at least 3 to 8 confirmations of different binding mode composed the interactions between ligands and targets (confirmations of the same ligand were merged together). The docking study confirmed the ERK-MAPK pathway, which may be potentially influenced by YHC, GKW and GRA. The active domains were confirmed by GeneCards (http://www.genecards.org/), Mg2+ and adenosine triphosphate (ATP) were coenzymes of MEK1.
