Table 2 Summary of differentially expressed proteins in EUU vs. EUK that were consistent in all antimicrobial susceptibility patterns.

From: Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation

Spot no.

Protein name

NCBI ID

Identification score (MS/MS ions score)

%Cov

No. of distinct matched peptides

pI

MW (kDa)

Spot intensity (arbitrary unit) (mean ± SEM)

Ratio (EUK/EUU)

P-value

EUU

EUK

27

Global DNA-binding transcriptional dual regulator H-NS

gi|15801465

1061

66

11

5.43

15.59

1.5950 ± 0.1713

0.8115 ± 0.0574

0.51

0.002

120

Elongation factor Tu

gi|15803852

1666

85

27

5.30

43.43

1.7768 ± 0.1559

3.5646 ± 0.2452

2.00

<0.001

135

Alkyl hydroperoxide reductase subunit F

gi|485774069

2551

65

29

5.47

56.50

0.0912 ± 0.0088

0.2367 ± 0.0203

2.60

<0.001

148

ATP-dependent chaperone protein ClpB

gi|487363647

2772

65

48

5.33

95.63

0.1231 ± 0.0274

0.2605 ± 0.0302

2.12

0.007

153

Pyruvate dehydrogenase E1 component

gi|545300671

2542

58

47

5.46

99.96

0.1662 ± 0.0201

0.4101 ± 0.0523

2.47

0.001

180

Acetate kinase

gi|15832434

1261

67

18

5.85

43.61

0.0000 ± 0.0000

0.1761 ± 0.0202

#DIV/0

<0.001

196

Phosphoenolpyruvate-protein phosphotransferase

gi|15802949

2146

69

37

4.78

63.72

0.3016 ± 0.0112

0.4619 ± 0.1014

1.53

0.048

201

Glycyl-tRNA synthetase beta subunit

gi|487363647

616

63

41

5.26

76.88

0.0866 ± 0.0097

0.1578 ± 0.0218

1.82

0.014

249

DNA-directed RNA polymerase subunit beta

gi|485809349

3463

53

69

5.15

150.95

0.0737 ± 0.0227

0.3758 ± 0.0649

5.10

0.002

  1. These common differentially expressed proteins were identified by nanoLC-MS/MS analysis.
  2. NCBI = National Center for Biotechnology Information
  3. %Cov = %Sequence coverage [(number of the matched residues/total number of residues in the entire sequence) × 100%]
  4. #DIV/0 = Divided by zero.