Table 2 Summary of differentially expressed proteins in EUU vs. EUK that were consistent in all antimicrobial susceptibility patterns.
Spot no. | Protein name | NCBI ID | Identification score (MS/MS ions score) | %Cov | No. of distinct matched peptides | pI | MW (kDa) | Spot intensity (arbitrary unit) (mean ± SEM) | Ratio (EUK/EUU) | P-value | |
|---|---|---|---|---|---|---|---|---|---|---|---|
EUU | EUK | ||||||||||
27 | Global DNA-binding transcriptional dual regulator H-NS | gi|15801465 | 1061 | 66 | 11 | 5.43 | 15.59 | 1.5950 ± 0.1713 | 0.8115 ± 0.0574 | 0.51 | 0.002 |
120 | Elongation factor Tu | gi|15803852 | 1666 | 85 | 27 | 5.30 | 43.43 | 1.7768 ± 0.1559 | 3.5646 ± 0.2452 | 2.00 | <0.001 |
135 | Alkyl hydroperoxide reductase subunit F | gi|485774069 | 2551 | 65 | 29 | 5.47 | 56.50 | 0.0912 ± 0.0088 | 0.2367 ± 0.0203 | 2.60 | <0.001 |
148 | ATP-dependent chaperone protein ClpB | gi|487363647 | 2772 | 65 | 48 | 5.33 | 95.63 | 0.1231 ± 0.0274 | 0.2605 ± 0.0302 | 2.12 | 0.007 |
153 | Pyruvate dehydrogenase E1 component | gi|545300671 | 2542 | 58 | 47 | 5.46 | 99.96 | 0.1662 ± 0.0201 | 0.4101 ± 0.0523 | 2.47 | 0.001 |
180 | Acetate kinase | gi|15832434 | 1261 | 67 | 18 | 5.85 | 43.61 | 0.0000 ± 0.0000 | 0.1761 ± 0.0202 | #DIV/0 | <0.001 |
196 | Phosphoenolpyruvate-protein phosphotransferase | gi|15802949 | 2146 | 69 | 37 | 4.78 | 63.72 | 0.3016 ± 0.0112 | 0.4619 ± 0.1014 | 1.53 | 0.048 |
201 | Glycyl-tRNA synthetase beta subunit | gi|487363647 | 616 | 63 | 41 | 5.26 | 76.88 | 0.0866 ± 0.0097 | 0.1578 ± 0.0218 | 1.82 | 0.014 |
249 | DNA-directed RNA polymerase subunit beta | gi|485809349 | 3463 | 53 | 69 | 5.15 | 150.95 | 0.0737 ± 0.0227 | 0.3758 ± 0.0649 | 5.10 | 0.002 |