Figure 3 | Scientific Reports

Figure 3

From: Synthesis, Chemical Characterization and Multiscale Biological Evaluation of a Dimeric-cRGD Peptide for Targeted Imaging of α V β 3 Integrin Activity

Figure 3

Colocalization between phycoerythrin-labeled anti- α V β 3 integrin primary antibody (PE-LM609) and FITC-labeled PEG4-cRGD2 probe. Human umbilical vein endothelial cells (HUVEC) were grown to confluency and incubated with both PE-LM609 and FITC-PEG4-cRGD2. Fluorescence microscopy images were acquired in DAPI/PE (A) and DAPI/FITC (B) channels and were superimposed to create colocalized pixel map (C) to calculate Pearson’s coefficient. Flow cytometric analysis of HUVEC co-incubated with PE-LM609 and FITC-PEG4-cRGD2 demonstrated a very high degree of colocalization between integrin α V β 3 and FITC-PEG4-cRGD2 probe (D).

Back to article page