Figure 4 | Scientific Reports

Figure 4

From: Synthesis, Chemical Characterization and Multiscale Biological Evaluation of a Dimeric-cRGD Peptide for Targeted Imaging of α V β 3 Integrin Activity

Figure 4

Binding kinetics of FITC and 64Cu labeled cRGD 2 probes. (A) Radioactivity of confluent HUVEC cells incubated with varying concentrations of 64Cu-NOTA-PEG4-cRGD2 (green) and with either 20 μM EDTA (red) or 50 μM of H-PEG4-cRGD2 (blue). (B) Fluorescence of the FITC-PEG4-cRGD2 (green) with either 20 μM EDTA (red) or 50 μM of H-PEG4-cRGD2 (blue). (C) Comparison of the fluorescence of the FITC-PEG4-cRGD2 (green) with FITC-Galacto-cRGD2 (orange). (D) Correlation between FITC and Cu64 labeled cRGD2 probes bound to HUVECs. (E) The fluorescence of HUVEC cells incubated with varying concentrations of FITC-PEG4-cRGD2 (green) and co-incubated with 20 μM of Mn2+ (purple). (F) Radioactivity of confluent HUVEC cells incubated with varying concentrations of 64Cu-NOTA-PEG4-cRGD2 (green) and co-incubated with 20 μM of Mn2+ (purple). (G) Competition binding between 64Cu-NOTA-PEG4-cRGD2 (50 nM) and increasing concentrations of unlabeled H-PEG4-cRGD2 in HUVEC cells.

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