Figure 6
Representative in vivo hybrid PET-CT reconstructed short-axis (SA), vertical- (VLA) and horizontal long-axis (HLA) images acquired with iodinated contrast agent (Omnipaque) at 90 min post-injection of 64Cu-NOTA-PEG4-cRGD2 (A). The iodinated blood pool contrast agent permitted better definition of right (RV) and left ventricle (LV) within the myocardium which is contoured with solid white line. Focal uptake of 64Cu-NOTA-PEG4-cRGD2 was seen within anteriolateral LV regions (dashed yellow arrow) although significant uptake was seen in chest wall (CH) at the thoracotomy site (solid yellow arrows) indicating active wound healing associated α V β 3 receptor expression. Bull’s eye myocardial plots of 64Cu-NOTA-PEG4-cRGD2 activity in diabetic and non-diabetic Lewis rats subjected to surgical ligation of LAD to induce myocardial infarction (arrow) (B). Hearts were immediately excised at 120 min post-injection, cleaned and filled with inert dental molding material and cut in four 2-mm thick slices from apex to base. After removing right ventricle (RV), each left ventricular (LV) slice was cut in four segments (anterior, septal, posterior, lateral), and 64Cu radioactivity was measured in each segment with gamma well counting. Data for each slice were expressed as percentage of injected dose per gram tissue (%I.D./g) and categorized as infarct, border and remote areas (C).
