Figure 2

Cell cycle. MCF7 and MDA-MB-231 cells were treated with 80 μM ω-3 FFAs and 20 μM ATRA alone or in combination for 24 h. (a) Cell cycle was analyzed by flow cytometry (upper panel) and percentages of the total cell population in the different phases of cell cycle. (b) The expression of p21, p27 and cyclin D1 protein and quantification of p21, p27 and cyclin D1 (relative to β-Actin).