Figure 3

Cell apoptosis. MCF7 cells were treated with 80 μM ω-3 FFAs and 20 μM ATRA. (a) Cell apoptosis was analyzed by flow cytometry with PI and Annexin V-FITC staining and percentage of apoptotic cells at two different stages. (b) PARP and Bcl-2 protein level after treatment from 12 h to 48 h. β-Actin was used as an internal control.