Figure 4 | Scientific Reports

Figure 4

From: RETRACTED ARTICLE: ω-3 free fatty acids and all-trans retinoic acid synergistically induce growth inhibition of three subtypes of breast cancer cell lines

Figure 4

p53 and Cell apoptosis. MCF7 and MDA-MB-231 cells were treated with 80 μM ω-3 FFAs, 20 μM ATRA alone or in combination for 48 h. (a) Relative expression of p53 was determined by Q-PCR. (b) The expression of p53 protein. β-Actin was used as an internal control. (c) Quantification of p53 protein level (relative to β-Actin). MCF7 and MDA-MB-231 cells were treated with 80 μM ω-3 FFAs and, 20 μM ATRA alone or in combination in the absence or presence of proteasome inhibitor MG132 or lysosome inhibitor CQ. (d) Cell counting assays. (e) The expression of PARP and p53 protein. β-Actin was used as an internal control. Values represent the mean ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.

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