Figure 9

Germination of hybrid seeds and ISSR validation. (a) A typical hybrid seed from crossing between clones 5504 and 7741. (b) Hybrid clones five days after germination, their female (F) and male (M) parents are used as size controls. (c) ISSR validation of the hybrid clones; M, molecular weight marker (Axygen 1 kb ladder); P1, male parent 7741; P2, female parent 5504; H1 through to H12, hybrid clones; the green arrow indicates 7741-specific band, the red arrow indicates 5504-specific band.