Table 1 Influence of different basic media and salicylic acid on the rate of mature flower formation.

From: Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile clone

Clone

Nutrient medium

Time to first flower emergence (d)

Flowering rate (%)

Lemna gibba 7741

E

n/a

0

H

n/a

0

MH

20

1.7 ± 0.5

E + 20 μM SA

10

51.0 ± 1.1

MH + 20 μM SA

12

73.5 ± 3.5

Lemna gibba 5504

E

n/a

0

H

n/a

0

MH

20

2.3 ± 0.8

E + 20 μM SA

14

6.5 ± 3.0

MH + 20 μM SA

12

54.1 ± 4.2

Lemna minor 7210

E

n/a

0

H

n/a

0

MH

n/a

0

E + 20 μM SA

n/a

0

MH + 20 μM SA

n/a

0

  1. Flower rate was evaluated 20 days after application of SA, the media were solidified with 0.6% agar. For the composition of the different Hoagland media E, H and MH cf. Table S1 (Supplementary information). Fronds were cultivated under 16 h light/8 h darkness. n/a = not applicable (no flowers occurred).
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