Figure 2

NRP-1 and PlGF tumor tissue expression associated with poor prognosis breast cancer. Representative immunohistochemical staining images for NRP-1 tumor tissue expression according to patient subgroups based on (a,b) nodal status, (c,d) metastatic status; and (e–g) NRP-1 and PlGF tumor expression in breast cancer patients tissues grouped according to their tumor molecular subtype. Graphs represent calculated mean immuno-reactive score (IRS) of NRP-1 and/or PlGF expression ± SEM in breast tumor tissue grouped according to the respective subtypes. NRP-1 tumor tissue expression was significantly higher in cases with metastasis and advanced nodal disease. Moreover, NRP-1 was significantly upregulated in TNBC cases compared to luminal B and luminal B-like; however, PlGF was significantly higher in TNBC cases compared to luminal B subtype only. p < 0.05 considered to indicate statistical significance. Scale bar = 50 µm, *p < 0.05, **p < 0.01, Multivariate ANOVA followed by Fisher’s LSD post hoc test.