Figure 5
Behavioural study in obese pre-diabetic mice subchronically treated with LH-21. (A–D) Exploratory activity was recorded for ten minutes in the open field maze in control, 45% HFD-vehicle and 45% HFD-LH-21 mice. Entries into center (A), time in center (B), distance in center (C) and overall distance travelled (D) were monitored. No changes among groups were detected. (E–G) Anxiety was analysed by the elevated plus maze test in these mice. Entries into open arm (E), time in open arms (F) and distance travelled in open arms (G) were recorded for five minutes. 45% HFD-vehicle mice showed an anxiety-like behaviour, with decreased time in open arms and distance travelled in open arms when compared to control mice. Subchronic treatment of obese pre-diabetic mice with LH-21 induced an anxiolytic-like behaviour with increased number of entries into open arms and LH-21 reverted the HFD-induced decrease in time in open arms and distance travelled in open arms. n = 8–10 mice each group. One-way ANOVA and Bonferroni’s post-test, *p < 0.05 versus Control-Vehicle, #p < 0.05 versus 45% HFD-Vehicle.
