Figure 6
Behavioural study in healthy mice acutely treated with LH-21 and CID16020046. (A) Exploratory activity was recorded for ten minutes in the open field maze in Veh-, LH-21- and CID + LH21-injected healthy mice. Entries into center, time in center, distance in center and overall distance travelled were monitored. Acute LH-21 injections tended to decrease exploratory activity in the center, and decreased total exploratory activity. These effects were totally reverted by CID16020046 pre-injection. (B) Anxiety was analysed by the elevated plus maze test in these mice. Entries into open arm, time in open arms and distance travelled in open arms were recorded for five minutes. LH-21-injected mice showed an anxiety-like behaviour with decreased entries and time into open arms when compared to control Veh-injected mice. These anxiogenic effects of LH-21 were totally reverted by CID16020046 pre-injection. n = 6–8 mice each group. One-way ANOVA and Bonferroni’s post-test, *p < 0.05 versus control; #p < 0.05, ##p < 0.01 versus LH-21-injected mice.
