Figure 2

DPP-4 gene and protein expression in osteoblasts isolated from controls and AIS patients. (a) DPP4 gene expression was measured by RT-qPCR with GAPDH as endogenous control (n = 7/group). Relative expression was analyzed with the 2^−ΔΔCT method. (b) DPP-4 protein expression was evaluated by Western blot (n = 3 controls and n = 6 AIS patients) and β-actin was used as endogenous control. **P < 0.01 using two-tailed Student’s t-test. Data are presented as mean ± standard deviation.