Figure 4 | Scientific Reports

Figure 4

From: Single-point Mutation of an Histidine-aspartic Domain-containing Gene involving in Chloroplast Ribosome Biogenesis Leads to White Fine Stripe Leaf in Rice

Figure 4

Map-based cloning of the WFSL1 gene. (a) Fine mapping of WFSL1. Markers used for mapping are indicated. AP002863 and AP002845 are accession of BACs. (b) Structure of WFSL1. ATG and TGA represent the start and stop codons, respectively. Black boxes indicate the exons and the lines indicate introns. A single nucleotide replacement ‘A’ to ‘T’ led to an amino acid change. (c) Leaf phenotype at tillering stage wild type and wfsl1 (left), complementation plants (COM) (middle), overexpression plants (OE) (right). (d) The chlorophyll content of wild type and complementation plants at tillering stage. (e) Sequencing results indicate wild type genome DNA fragment transfer into wfsl1 plants. (f) The expression level of wild type, wfsl1, complementation and overexpression at tillering stage. The relative expression level of wfsl1 represents “1” Data are mean Âħ SD (n = 3). Bars: (c) 5 cm. Error bars represent the SD from three independent experiments.(Student’s t-test, *P < 0.05; **P < 0.01)

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