Figure 1

Three monophylic clusters identified among eukaryotic Ca2+ activated protein kinases. (A) The rooted tree displaying three monophylies of Ca2+ activated protein kinases among the eukaryotes. Mitogen-activated protein kinase (MAPK) was the outgroup. Major nodes were shown with two supporting values: maximum-likelihood by FastTree (upper number), randomized axelerated maximum likelihood methods (lower number). Branches are colored to represent different origins, Green: Archaeplastida, blue: Opisthokonta, red: SAR, black: Amoebozoa, purple: Excavata. (B) Schematic representation of monophylic cluster specific structural features with the schematic structures from the first and the last branch. Yellow block: kinase domain, red block: EF hand, green bar: monophyly specific inserted amino acid (s).