Figure 3

(a) Impact of fungal infection on blood-feeding at 1–5 days post-infection with either Met-Hybrid or Met-RFP. Mosquitoes were placed in a choice chamber with the guinea pig host outside of the chamber and just out of reach (Supplementary Figure 1a,b). Host-seeking (blood feeding) interest was quantified as the percentage of the mosquito population choosing the chamber closest to the host. The symbol “*” denotes no significant differences in mosquito choices with or without a host: 30 ± 3.05% of the mosquitoes chose the “host” chamber even in the absence of a host. (b) Mortality and transmission of mosquitoes exposed to fungus, the light area represents the percent survival of mosquitoes for each treatment, while the dark area shows the proportion of mosquitoes in each treatment that are alive and would seek a host to blood feed (capable of malaria transmission). The upper dashed line represents the LT50 while the lower dashed line represents the 80% control threshold suggested by the World Health Organization (WHO) for a successful vector control agent¹⁸.