Figure 1

Hypoxia down-regulates miR-125a in HCC cells and tumors. (a,b) Relative levels of 22 miRNAs in HepG2 and Huh-7 cells after treatment with hypoxia for 48 h were measured using RT-qPCR. (c) Relative levels of miR-125a (expressed as the miRNA/U6 ratio) in paired tumor tissues and normal tissues were determined using RT-qPCR (n = 21). (d) Localization of miR-125a in paired tumor tissues (HCC) and normal tissues (Normal) from HCC patients were analyzed by in situ hybridization. All data are shown as the means ± S.E. obtained from three separate experiments.