Figure 3
miR-125a directly targets HK2. (a) A schematic diagram of HK2 3′UTR as a putative target for miR-125a. The seed-recognizing sites and mutant sites are indicated in red. (b) Relative luciferase activity in 293T that were transfected with firefly luciferase reporters containing WT or mutant 3′ UTRs of HK2, miR-125a mimics or miR-NC. (c–f) Huh-7 and HepG2 cells were transfected with miR-NC, miR-125a mimics, anti-miR-NC or anti-miR-125a. The expression levels of HK2 were detected using immunoblotting.
