Figure 5
The miR-125a/HK2 axis is functionally important for regulating HCC cell glucose metabolism, growth and apoptosis. HepG2 were transfected with miR-NC, miR-125a mimics, HK2 vector and miR-125a mimics plus HK2-vector in normaxia. (a) Representative Western blotting analyses of HK2. The uptake of glucose (b), the production of lactate (c), cellular levels of ATP (d) and ROS (e) were evaluated. (f) ROS was observed by fluorescence micrography. (g) Apoptosis of HepG2 cells under various transfections was analyzed using flow cytometry. (h) Viabilities of HepG2 cells under various transfections were determined using a CCK-8 assay at various time points.
