Figure 6
The miR-125a/HK2 axis is of functional importance in regulating the Warburg effect in vivo. Mice were divided into four groups according to the implanted HepG2 cells: control cells (CTL), miR-125a-overexpressing cells (miR-125a), HK2-overexpressing cells (HK2), and HK2 plus Lenti-miR-125a cells (HK2 + miR-125a). (a) Representative images of tumors. (b) The time course of tumor volume. (c) The quantitative analysis of tumor weight. (d) Western blotting analyses of HK2 proteins in tumors from four groups of mice. The metabolic parameters of the tumors from four groups was measured, the uptake of glucose (e), the production of lactate (f), cellular levels of ATP (g), and ROS (h,i). (j) HE staining, HK2 and HIF-1α immunohistochemical staining, Ki67 and Tunel staining of tumor tissues in four groups of mice. All data are shown as the means ± S.E. obtained from three separate experiments.
