Figure 5 | Scientific Reports

Figure 5

From: Targeted sequencing of both DNA strands barcoded and captured individually by RNA probes to identify genome-wide ultra-rare mutations

Figure 5

SNV-calling trends and statistics of DEEPER-Seq WES study. (A) Total number of SNVs detected at increasing read count thresholds. Sensitivity increases at higher read counts but quickly reaches a plateau with more than 80 million reads. (B) Average SNV frequencies of normal tissue DNA measured by three approaches: a standard NGS approach where barcodes were directly trimmed off, a super read based approach by DEEPER-Seq without matching variants from both DNA strands (without the last step of the 4-step procedure), and a super read approach by DEEPER-Seq matching the SNV on both strands (all steps in the 4-step procedure were performed).

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