Table 1 Data yield from DEEPER-Seq WES sequencing.

From: Targeted sequencing of both DNA strands barcoded and captured individually by RNA probes to identify genome-wide ultra-rare mutations

 

Normal Tissue

Tumor Tissue

Initial mapped reads

30.7 billion

25.7 billion

Average raw coverage

240.5×

173.9×

Unique read family (URF)

625 million

577 million

Super read duplexes

272 million

231 million

Initial mapped reads per super read family

49.1

44.5

Initial mapped reads per super read duplex

112.9

111.3

Super reads per super read duplex

2.3

2.5

  1. Data yield from DEEPER-Seq WES sequencing. Initial mapped reads represent raw reads that contain the 12nt barcode and mapped to the reference genome. Unique read family represents the number of URF. Each URF has a unique barcode and its sequence is obtained by consolidating read sequences arise from the same DNA molecule by PCR amplification. PCR errors are removed by requesting a sequence uniformity for over 95% of the reads within a URF. Super read duplexes represent the number of DNA duplex whose two strands are coming from two super reads.
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