Figure 2

NR data and SLD profiles for POPC supported bilayers before and after d-p7 incorporation. (A) NR data and fits for the bilayer measured in D2O (red circles), CMW (green squares), and H2O (blue triangles). Data is presented multiplied by q z 4. (B) Data and fits after d-p7 incorporation. For comparison, dotted lines are the fits before protein incorporation. Solid lines are fits using a cylindrical protein model with a uniform SLD distribution spanning the bilayer. Dashed lines are the best fits to the H2O data without protein (below) and with protein but without water penetration (above). (C) SLD profiles before (dotted lines) and after (solid lines) d-p7 incorporation. The schematic indicates the location of bilayer components and quartz interface (z = 0). (D) Magnification of the lipid tails region shows increased SLD, particularly in the H2O contrast, indicating incorporation of d-p7 into the bilayer.