Figure 3 | Scientific Reports

Figure 3

From: Coupling neutron reflectivity with cell-free protein synthesis to probe membrane protein structure in supported bilayers

Figure 3

NR data and SLD profiles for asolectin bilayers before and after incorporation of either h-p7 or d-p7. (A) Data and fits for the bilayer measured in D2O (red circles), CMW (green squares), and H2O (blue triangles). Data is presented multiplied by q z 4. (B) Data and fits after h-p7 incorporation using a cylindrical protein model with a uniform SLD distribution spanning the bilayer. (C) Data and fits after d-p7 incorporation. Dashed lines are best fits using a cylindrical protein model and solid lines are fits using a conical protein model. (D) SLD profiles of the asolectin bilayer before (dotted lines) and after (solid lines) h-p7 incorporation corresponding to the fits in panels A and B. The quartz interface is at z = 0. (E) SLD profiles before (dotted lines) and after (solid lines) d-p7 incorporation corresponding to the fits in panel A and the conical protein model fits in panel C.

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