Figure 2
Distribution of distances between lacO loci. Histograms of the distances between the two lacO spots in each developmental stage. Sample sizes are indicated in Supplementary Table S3 (“the number of pairs of lacO spots”). Black solid lines indicate the expected distribution of the distances if the two spots were randomly positioned in a nucleus of the same size, predicted from a Monte Carlo simulation. The average sizes of the nuclei at each stage are described under “radius” in Supplementary Table S3. Thick dotted lines indicate the upper and lower limits of the 95% confidence interval calculated from a random simulation with the number of pairs equal to that of the lacO spots for each stage as described under “the number of pairs of the lacO spots” in Supplementary Table S3 (see Methods). Similarly, thin dotted lines indicate the limits when the number of random pairs was the same as the number of nuclei examined as described under “the number of nuclei” in Supplementary Table S3. The latter corresponds to an extreme situation where the two spots do not move at all in each nucleus, and thus the expected variation in the distribution is large. Red arrows indicate where the experimentally obtained distribution did not agree with the random position scenario. Differences in the distributions among different stages are more directly compared in Fig. S3, where the distances between the lacO spots were normalised by the radius of the nucleus.
