Figure 7
Influence of 18s rRNA gene blocking strategies for taxonomic richness and evenness. Alpha-diversity boxplots for the three samples of the analysis panel under different amplification conditions in the V4 (orange) and V9 (blue) hypervariable regions. Each boxplot represents 100 estimates of alpha-diversity (richness: observed species, top plot; and evenness: Shannon index, bottom plot) computed from 100 random subsamples of size 1000 in each amplicon dataset. Whiskers represent values within 1.5x of the inter-quartile range, from the third (upper whisker) and first (lower whisker) quartiles of the alpha-diversity distribution. Outlier values beyond the end of the whiskers are plotted as points.
