Figure 1
From: Regulation of retinal pigment epithelial cell phenotype by Annexin A8
Suppression of AnxA8 phenocopies FR in RPE cells. (A) Immunofluorescence staining for AnxA8 (green) is decreased in response to both FR (7 days) and AnxA8 siRNA in ARPE-19 cells whereas vehicle (DMSO) and control siRNA had no effect. Nuclei were stained with DAPI (blue), and rhodamine phalloidin (red) revealed cell extensions in FR- or AnxA8 siRNA-treated cells (yellow arrows in insets). Experiments were performed three times and representative images are shown, scale bar = 50 µm. (B) Expression levels of AnxA8 were analysed with real-time PCR. Both FR and AnxA8 siRNA elicited significant reductions in the level of AnxA8 mRNA. Shown are mean and standard error of five independent experiments. Student’s t-test was used to calculate statistical significance (***=P < 0.001).
