Figure 2
From: Regulation of retinal pigment epithelial cell phenotype by Annexin A8
Primary porcine RPE respond to FR and AnxA8 siRNA in the same way as ARPE-19 cells. (A) FR and AnxA8 siRNA treatment for 7 days results in fewer cells (DAPI, blue), formation of cytoplasmic extensions and F-actin rich domains at the cell borders (rhodamine phalloidin, red) with concomitant down-regulation of AnxA8 (green). Scale bar = 50 μm. Shown are representative immunofluorescence images from 3 independent experiments. (B) Cell lysates were prepared under each of the conditions shown in (A) and AnxA8 siRNA levels were quantified by real-time PCR. Similar to ARPE-19 cells, porcine AnxA8 mRNA transcripts were down-regulated in the presence of FR and AnxA8 siRNA. Data show mean and standard error from 5 independent experiments. Student’s t-test was used to calculate statistical significance (***=P < 0.001). (C) Representative phase images show that FR and AnxA8 siRNA treatment both lead to extension formation and suppression of cell growth as observed in ARPE-19 cells. Scale bar = 100 μm.
