Table 2 Human and porcine primer pairs used for real-time PCR. Shown are the forward (F) and reverse (R) sequence of the primers, as well as PCR conditions.
From: Regulation of retinal pigment epithelial cell phenotype by Annexin A8
Target |  | Sequence from 5′ to 3′ | Initial step | Denaturation, annealing, elongation | Cycles |
|---|---|---|---|---|---|
huAnxA8 | F | TGG GAC CCT GAT AAG AAA CAT | 10 min 95 °C | 15 sec 95 °C, 1 min 60 °C | 40 |
R | TCC TGG AGA CTC TGG CTT CAT | ||||
huCalbindin | F | CCT GCT GCT CTT CCG ATG CCA G | |||
R | GGT AGT AAC CTG GCC ATC TCA G | ||||
huCalretinin | F | CCT GCA CCT GGC CGA GCT GAC G | |||
R | GCT GGA GCC CAC GTG CTG CCT G | ||||
huGAPDH | F | ACC CAC TCC TCC ACC TTT G | |||
R | CTC TTG TGC TCT TGC TGG G | ||||
porcAnxA8 | F | CCC AGA CCC CGA CGC GGA GAC C | |||
R | GTT CTT GGT CCG AGA AGC CAG G | ||||
porcGAPDH | F | AAG TGG ACA TTG TCG CCA TC | |||
R | TCA CAA ACA TGG GGG CAT C |
