Figure 4 | Scientific Reports

Figure 4

From: Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy

Figure 4

3D segmentation of the LCN. 3D reconstructions of a 50 × 50 × 20 μm3 volume of a bovine femur imaged with THG (a) and confocal fluorescence (CLSM, b). (c) (resp. (d)), skeletonization of the segmented signal shown in (a) (resp. (b)). (e) Comparison of the LCN skeleton calculated from fluorescence (CLSM, left) and THG (middle) images. The overlay of the two retrieved skeletons (right) highlights the consistency of the results obtained with the two techniques. Scale bar, 10 µm.

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