Figure 5
Imaging mouse bone structures at the organ scale. Whole section of a mouse femur imaged a few microns below the sample surface with THG (a) and SHG (b). Tubular structures of diameter consistent with that of vascular channels (~11 ± 3 µm), appear either with a positive (white arrows) or negative (yellow arrow) contrast in the THG images. Scale bar, 500 µm. Total imaging time, 333 s (including overlaps between images). (c) (resp. (d)), zoom into the red rectangle area in (a) (resp. (b)), showing thin and elongated lacunae along the tangential direction, i.e. parallel to the endost and periost (green arrows), canaliculi mainly oriented along the radial direction (red arrows) in the THG image, and a collagen lamellar periodic structure in SHG (orange arrow). Scale bar, 20 µm. (e) (resp. (f)), zoom into the orange rectangle area in (a) (resp. (b)), showing a vascular channel, rounder lacunae (blue arrows) and a more disordered collagen structure.
