Figure 7
Effect of the sample preparation on THG imaging. THG images of (a), dehydrated; (b), fixed; (c), decalcified; and (d), resin-embedded transverse sections within the same bovine tarsis. Dehydrated and fixed samples yield similar THG images, while significant differences appear for decalcified and PMMA-embedded samples: in the former case, the modulation anticorrelated with the SHG signal is more pronounced and the cement line gives rise to a positive contrast. In the latter, the contrast of the canaliculi is reduced. Scale bars, 50 µm. Insets, zooms into the orange squares. Scale bars, 10 µm.
