Figure 8 | Scientific Reports

Figure 8

From: Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy

Figure 8

Segmentation of bone structural units using THG images. (a) Mosaic THG image of a human femoral cortical bone exhibiting extensive remodeling. (b) Zoom into the red rectangle in (a) showing the dip in the THG signal used to segment BSUs (red arrows). (c) Segmented THG with the different BSUs displayed using different colors. (d) Corresponding SHG image. Scale bars, 200 µm in (a–c); 50 µm in (b).

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