Figure 9
Combined fluorescence/THG/SHG imaging. THG (a) and SHG (b) images of the iliac crest of an ewe. In (a), THG is shown in purple and fluorescence in orange. The white arrow indicates a location where calcein was incorporating during tissue formation. Scale bars, 500 µm. (c) (resp. (d)), zoom into the white rectangle in (a) (resp. (b)). Scale bars, 50 µm. (e), THG (blue), SHG (green) and fluorescence (orange) profiles along the yellow box in (c,d), showing the anticorrelation between THG and SHG signals. The modulation of the THG signal is of much smaller amplitude than that of the SHG signal.
