Figure 5

Confocal micrographs showing localization of the α1, β1, δ, ε, and γ subunits at the neuromuscular junctions (NMJs) of the (A) gastrocnemius and (B) normal orbicularis oris. Immunofluorescence staining with α-bungarotoxin (green, mark α1 subunit), β1 or δ or ε or γ subunits (pink), and neurofilament 200 (red, mark nerve). Arrows mark the NMJs. Scale bars = 30 μm.