Figure 1 | Scientific Reports

Figure 1

From: Aerobic biogenesis of selenium nanoparticles by Enterobacter cloacae Z0206 as a consequence of fumarate reductase mediated selenite reduction

Figure 1

Growth and selenite reduction of E. cloacae Z0206 in the presence of various concentrations of selenite. (A) Apparent changes in spent broth. (B) Growth profile under different selenite concentrations. Samples of 1 ml of the bacterial culture were collected at different time intervals of bacteria growth and then centrifuged at 4 °C and 10,000 × g for 10 min. Protein was extracted from the pellet using a total bacterial protein extraction assay kit. Bacterial growth was measured via the quantification of total cell protein. The protein concentration in bacteria cell extracts was determined. (C) Quantity of bacteria at the time point of 96 h. (D) Dynamic changes in selenite residue in the broth. (E) Kinetic study of selenite reduction by E. cloacae Z0206 under different selenite concentrations. Line graphs and bar graphs are presented as the mean ± SD, **P < 0.01 (n = 3). (F) SEM analysis of E. cloacae Z0206 under different selenite concentrations. The bacteria were cultured in the presence of various concentrations of selenite (0 mM, 0.5 mM, 1 mM, 5 mM, 10 mM and 15 mM). Samples were collected when the selenite was completely consumed according to the results in Fig. 1D.

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