Figure 5

Involvement of the chitin metabolic pathway and working model underlying the basis of desiccation tolerance. (A) CDA enzyme activity. Data represented are mean ± SD of eight replicates (a = p < 0.05 vs 3 h rehydration). (B) Fold induction of cda transcripts. Data represented are mean ± SD of eight replicates (a = p < 0.05 vs 3 h rehydration). Undesiccated control values were set to 1 against which all other values were compared. The results were represented as fold induction relative to tubulin. (C) Chitosanase enzyme activity. Data represented are mean ± SD of eight replicates (a = p < 0.05 vs 5 h rehydration). (D) Chitin synthase enzyme activity. Data represented are mean ± SD of eight replicates (a = p < 0.05 vs 4 h rehydration). (E) Fold induction of chs transcripts. Data represented are mean ± SD of eight replicates (a = p < 0.05 vs 4 h rehydration). Undesiccated control values were set to 1 against which all other values were compared. The results were represented as fold induction relative to tubulin. (F) Schematic depicting the collaborative interplay of key biomolecular players namely, trehalose, chitin and glucosamine implicated during desiccation tolerance in C. ramosus.