Figure 7 | Scientific Reports

Figure 7

From: Enhanced chondrogenesis of bone marrow-derived stem cells by using a combinatory cell therapy strategy with BMP-2/TGF-β1, hypoxia, and COL1A1/HtrA1 siRNAs

Figure 7

Effects of COL1A1 and/or HtrA1 siRNA on collagen and HtrA1 synthesis. hBM-MSCs were cultured under hypoxia with or without BMP-2/TGF-β1 (B-T), and transfected with NC, or COL1A1 and/or HtrA1 siRNAs in type I collagen sponges for 14 days, as described in Fig. 6. Protein extracts from treated-MSCs, undifferentiated MSCs at day 0 (D0), and a macroscopically healthy zone of human OA cartilage were analyzed by Western-blotting for type I, II, and X collagens, and HtrA1 versus GAPDH. Different exposures of the blots are provided in the supplementary information file (Fig. S6). Corresponding quantifications of the bands of interest, normalized to the GAPDH signals, are indicated as arbitrary units below each blot. Representative blots are shown.

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