Figure 1 | Scientific Reports

Figure 1

From: Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana

Figure 1

Phytohormones affect the expression level of mature miR166/165. The expression level of miR166/165 was quantified using stem-loop qRT-PCR in roots at 1, 6, 12, and 24 hrs after treatment with (A) 10 µM IAA, (B) 10 µM GA, (C) 10 µM ABA, (D) 10 µM BAP, (E) 20 µM JA, and (F) 100 µM SA. Whole mount in situ localization of miR166/165 was performed in 7 dag roots following 12 hrs of phytohormone treatment (G–J). (G) Control (without any treatment), (H) 10 µM IAA, (I) 10 µM ABA and with (J) 20 µM JA. In situ localization of miR166/165 in BAP, GA and SA treatment has been provided in Supplemental Fig. S3. Error bars indicate ± SE of two independent biological experiments. One-way ANOVA was performed. Statistically significant differences are indicated as, * for P < 0.05, ** for P < 0.01 and *** for P < 0.001. Scale bar indicates 50 µm.

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