Figure 5

(A) Plot of the change in average 1HN and 15N chemical shifts following titration of 15N-RXFP1(1–72) P4F with 50 equivalents of truncated relaxin. (B) Single-site saturation binding curves (Kd = 330 ± 10 μM) for the resonances that show the largest chemical shift changes and remained resolved throughout the titration.