Figure 1
From: Methylation of DACT2 promotes breast cancer development by activating Wnt signaling
Expression of DACT2 was silenced by DNA methylation in breast cancer cell lines (A) RT-PCR shows the levels of DACT2 expression. MDA-MB-231, BT474, MDA-MB-468, HBL100 and BT549 are breast cancer cell lines. (−) untreated; (+) 5-Aza treated; H2O: double distilled water. GAPDH was used as an internal control. (B) MSP results of DACT2 in breast cancer cell lines. IVD: in vitro methylated DNA; NL: normal lymphocyte DNA; M: methylated alleles; U: unmethylated alleles. (C) Bisulfite sequencing results: Double-headed arrow indicates the region of the MSP product. Filled circles: methylated CpG sites; open circles: unmethylated CpG sites. TSS: transcription start site.
