Figure 5
From: Methylation of DACT2 promotes breast cancer development by activating Wnt signaling
The role of DACT2 in Wnt/β-catenin signaling and the effects of DACT2 on breast cancer cell line xenografts (A) Active-β-catenin, p-β-catenin, total β-catenin, myc, cyclin D1 and cyclin E1 were detected by western blot in DACT2 unexpressed (vector) and re-expressed (DACT2) MDA-MB-468 and HBL100 cells. The results were validated by knocking down DACT2 in MDA-MB-231 cells. (B) Representative results of DACT2 re-expressed and unexpressed MDA-MB-468 cell xenografts in mice. V: control group; D: DACT2 re-expressed breast cancer cells group (C) Tumor growth curves of DACT2 re-expressed and unexpressed MDA-MB-468 cell xenografts. ***P < 0.001 (D) The average weights of DACT2 re-expressed and unexpressed MDA-MB-468 cell xenografts. ***P < 0.001
