Figure 4

Infectious properties of WNV RRPs in Vero, HEK-293, BHK-21 and SK-N-SH cells. (a) Expression of the GFP reporter gene in each cell line after the infection of equal amounts of RRPs. (b) Monolayers of each cell line in a six-well plate containing 105 to 106 cells/well were infected in parallel with 10-fold serial dilutions of WNV RRPs. Cells positive for GFP protein were counted at the appropriate dilution and the number of RRPs per 1 mL of supernatant were calculated.