Figure 6
Propagation characteristics of RRPs in BWNV-CME and BHK-21 cells. (a) The spread of green fluorescence in BWNV-CME cells infected with RRPs. In RRP-infected BHK-21 cells, the green fluorescence expressing cells did not increase from 24 h post-infection. The BWNV-CME and BHK-21 cells were seeded into 24-well plates and inoculated with RRPs for the indicated time periods. For detection of plaques, the infected cells were overlaid with Eagle’s medium containing 1.5% carboxymethyl cellulose and 1% foetal calf serum. The morphological changes of one plaque were observed with a fluorescent microscope. (b) WNV plaque formation in WNV-CME cells. Six days post-infection, the plates were stained with 0.1% crystal violet solution for 15 min at room temperature. The stain was discarded and the cells were rinsed with tap water.
