Figure 2
Lung tissue inflammation and structural airway remodeling is enhanced by IL-33 administration in OVA-sensitized mice. (a) Scoring of inflammation in lung tissue. 0 = no inflammatory cell infiltration, 1 = inflammatory cells present in central airways, 2 = inflammatory cells present in central airways and parenchyma, 3 = massive infiltration of inflammatory cell in the central airways as well as in the parenchyma. (b) Representative images of haematoxylin and eosin staining of lung tissue graded in panel 2a. (c) Scoring of the ASM cell layer around the central airways. The thickness of the ASM cell layer was assessed and a relative score was assigned to each sample, where 0 = normal, 1 = thickened and 2 = substantially thickened ASM cell layer. ASM = airway smooth muscle. (a,c) *p < 0.05 (Chi-square test for trend). Results are pooled data from four independent experiments (mean ± SEM of n = 9–10 mice for each group). (d) Relative quantitation (RQ) of mRNA levels of Col1a1, Col3a1 and Col5a1 were assessed in lung specimens. *p < 0.05, **p < 0.01 (ANOVA, Bonferroni). Results are pooled data from four independent experiments (mean ± SEM of n = 3–4 mice in each group).
